Experiment 1 Polymerization of acrylamide in water

APE was used as crosslinking agent 2 5 g of acrylamide 2 5 g of acrylic acid and 50 mg of APE were dissolved in 20 mL methanol and then 0 2 0 4 0 6 0 8 1 g of DMAm were added to the alcoholic solution of AA/AAm (Table 1) The polymerization was initiated by the addition of 1 mL of 5% (w/v) solution of BPO in methanol After bubbling nitrogen for 15 min the reaction was carried out in 2 1 Materials The Acrylamide AA (stated purity 98%) was and 75/25 and 0 0001 mmole of AIBN were placed into a pyrex flask The polymerization was carried out at 60C for 1 hour under argon atmosphere At the end of it still cannot adsorp the dye because dye cannot compete with water But the experiment with copolymer K3 gave 50%

Kinetics and Molecular Weight Control of the

For RAFT polymerization of acrylamide under these experimental conditions a continuing supply of radicals was required in order to achieve reasonable conversions The deviations of experimentally measured molecular weights from those theoretically predicted are a function of the CTA utilized and parallel the extent of rate retardation The deviations are at least in part consistent with

Per cassette we mix 2 5 ml buffer stock and sufficient acrylamide stock so that when the mix is brought to final volume with distilled water we have the desired percent acrylamide monomer Acrylamide polymerizes spontaneously in the absence of oxygen so the polymerization process involves complete removal of oxygen from the solution

There are two types of polymerization known as step-reaction polymerization or condensation and chain-reaction polymerization These two types of polymerization both create high molecular mass molecules from monomers through a chemical reaction With chain-reaction polymerization there are three steps that takes two chemicals

Experiment 1: Polymerization of acrylamide in water Aim: (a) To polymerize acrylamide (monomer) in water to polyacrylamide (polymer) by free radical polymerization using a redox pair of initiators (b) To follow the kinetics of polymerization by monitoring the variation of The polymerization of acrylamide in water solution is of interest

the monomer acrylamide the cross-linking agent methylenebis-acrylamide and a free radical generator ammonium persulfate in aqueous buffer (Figure 2) Free radical polymerization of the acrylamide occurs At various points the acrylamide polymers are bridged to each other

polymerization of acrylamide temed

TEMED is used with ammonium persulfate to catalyze the polymerization of acrylamide when making polyacrylamide gels used in gel electrophoresis for the separation of proteins or nucleic acids Although the amounts used in this technique may vary from method to method 0 1–0 2% v/v TEMED is a traditional range

Figure 1 (A) Polyacrylamide gel formation Acrylamide and bis are copolymerized in a reaction catalyzed by ammonium persulfate and TEMED (B) Hydrolysis of acrylamide to acrylate Pendant neutral carboxamide groups can hydrolyze to charged carboxyls For protein electrophoresis the pores of the gel are the important structures

2925cm-1 is assigned to –C-H stretching of the acrylate group The peak at 1676cm-1 and at 1726 cm-1 are assigned to C=O stretching of the acrylamid groups and acrylate groups respectively The peak at 1168cm-1 is attributed to –COO stretching of the acrylate group and the peak at 1559 cm-1

mass gmol 1 density g/cm 3 solubilityinwater: g/mL at C serves as chain transfer agent in the copolymerization process) potassium persulfate K 2 S2 O 8 (molar mass gmol 1 density g/cm 3 solubility in water: g/mL at C serves as initiator in the copolymerizationprocess) For the preparation of acrylamide-acrylic acid monomer solution

1 mM Dilute in water Do not re-use once defrosted Preparation of lysate from Polyacrylamide gels are formed from the polymerization of two compounds acrylamide and N N-methylenebis-acrylamide (Bis for short) Bis is a cross-linking agent for the gels The polymerization is initiated by the addition of ammonium persulfate along with

A semi-continuous synthesis of polystyrene/acrolein latex was carried out The optimum addition time of acrolein monomer was determined to be 10 hours after addition of the initiator giving a monodisperse polystyrene latex with aldehyde on the surface A DNPH assay was used to assay the aldehyde group in the latex suspension it was specifically a surface assay It successfully detected

1 mM Dilute in water Do not re-use once defrosted Preparation of lysate from Polyacrylamide gels are formed from the polymerization of two compounds acrylamide and N N-methylenebis-acrylamide (Bis for short) Bis is a cross-linking agent for the gels The polymerization is initiated by the addition of ammonium persulfate along with

Experiment 1: Polymerization of acrylamide in water The polymerization of acrylamide in water solution is of interest because the rate constant kp for the propogation reaction I is unusually large the rate constant for termination relatively low the so called transfer processes

Anionic Acrylamide Polymer for Lebanon

It increases the viscosity of water and facilitates the flocculation of particles present in water Anionic Poly-Acrylamide (aPAM) projects ncsu edu ANIONIC POLYACRYLAMIDE Composition: Negatively charged copolymers of acrylamide are widely used as retention aids and dry-strength resins However different molecular mass ranges are used for these two roles Anionic acrylamide copolymer

n!1 10) were obtained in the case of t-BDB [32] Since RAFT appears to be more efficient than NMP [33– 36] and ATRP [37–40] concerning CRP of acrylamide monomers [41–50] it was chosen to copolymerize NAS with another bi-substituted acrylamide derivative dimethy-lacrylamide (DMA) Poly(DMA) is a nonionic water-

Abstract In this work sugarcane bagasse being an abundant and renewable resource was used as a raw material to prepare sugarcane bagasse-g-poly(acrylic acid-co-acrylamide) (SB/P(AA-co-AM)) hydrogels The hydrogels were prepared by free radical graft copolymerization of SB with AA and AM using N N-methylene-bis-acrylamide (MBA) as a crosslinker The optimal synthesis conditions were

The polymerization reaction creates a gel because of the added bisacrylamide which can form cross-links between two acrylamide molecules The ratio of bisacrylamide to acrylamide can be varied for special purposes but is generally about 1 part in 35 15

the monomer acrylamide the cross-linking agent methylenebis-acrylamide and a free radical generator ammonium persulfate in aqueous buffer (Figure 2) Free radical polymerization of the acrylamide occurs At various points the acrylamide polymers are bridged to each other

nium persulfate to prevent polymerization while pouring the gel If particulate matter remains filter through a Whatman no 1 filter paper in a funnel To achieve slower polymerization reduce amounts of TEMED and ammonium persulfate to 40 L and 0 4 mL respectively 5 Pour gel immediately Gently pull acrylamide solution into a 60-mL syringe

Atom transfer radical polymerization (ATRP) of acrylamide has been carried out in water or in glycerol−water (1:1 v/v) medium at 130 C using water-soluble initiators viz 2-chloropropionamide (2-Cl-PA) or 2-bromopropionamide (2-Br-PA) and CuX (X = Cl Br) bipyridine complex as catalyst Extraneous addition of CuX2 (20 mol % of CuX) and/or excess X- ions (1 M alkali halide) in the reaction

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