Scholarly articles for in vitro enzymatic assay

The psychoactive cannabinoids from Cannabis sativa L and the arachidonic acid-derived endocannabinoids are nonselective natural ligands for cannabinoid receptor type 1 (CB1) and CB2 receptors Although the CB1 receptor is responsible for the psychomodulatory effects activation of the CB2 receptor is a potential therapeutic strategy for the treatment of inflammation pain atherosclerosis A method for obtaining sequence information directly from plasmid DNA is presented The procedure involves the rapid preparation of clean supercoiled plasmid DNA from small bacterial cultures its complete denaturation by alkali and sequence determination using oligodeoxyribonucleotide-primed enzymatic DNA synthesis in the presence of dideoxynucleoside triphosphates

Bentham Science

Our peer-reviewed scholarly journals and books have an ever-increasing readership of millions of researchers worldwide Bentham Science currently publishes more than 100 journals in both electronic and printed formats Our journals cover various disciplines in pharmaceutical research and development medical subspecialties engineering technology and social sciences The journals are indexed

Growing evidence shows that gut microbes are key factors involved in the regulation of energy homeostasis metabolic inflammation lipid metabolism and glucose metabolism Therefore gut microbiota modulations caused by selectively fermented oligosaccharides or probiotic bacteria constitute an interesting target in the physiopathology of obesity

Currently an increasing demand of cannabis-derived products for recreational and medical use is observed Therefore the reliable and fast quantification of cannabinoids in hemp samples is essential for the control of product from Cannabis sativa L strains In general gas chromatography (GC) is the method of choice for the quantification of cannabinoids whereas this method is time

Continuous glucose monitoring (CGM) is well established in the management of diabetes mellitus but its role in neonatal glycaemic control is less clear CGM has provided important insights about neonatal glucose metabolism and there is increasing interest in its clinical use particularly in preterm neonates and in those in whom glucose control is difficult

Biochemical Society and Portland Press suspend paywalls on all published content In light of the COVID-19 pandemic and the inability of most researchers across the World to access their laboratories the Biochemical Journal wishes to emphasize its long-standing painless publishing strategy To help limit delays in dissemination of important work and our progress as an academic community we

White Blood Cell Function: Overview of the Immune System

12 08 2016T-cell activation may be assessed in vitro by mitogen-response testing T-cell activation and proliferation in response to various mitogens may provide useful information with respect to T-cell function Phagocytes may be enumerated with a WBC count and differential Phagocytic activity can be evaluated using a respiratory-burst assay with fluorescent dyes or the older NBT test (see Chronic

SUMMARY Toxoplasma gondii is an obligate intracellular pathogen within the phylum Apicomplexa This protozoan parasite is one of the most widespread with a broad host range including many birds and mammals and a geographic range that is nearly worldwide While infection of healthy adults is usually relatively mild serious disease can result in utero or when the host is immunocompromised

Education Center - Introductory - Topics in Plant Pathology - Overview of Plant DefensesBrian C Freeman and Gwyn A BeattieIowa State University Freeman B C and G A Beattie 2008 An Overview of Plant Defenses against Pathogens and Herbivores The Plant Health Instructor DOI: 10 1094/PHI-I-2008-0226-01 Introduction Plants represent a rich source of nutrients for many or

Enolase is a key enzyme in energy metabolism and measuring its enzymatic activity in vitro is of interest to investigators in diverse fields including those studying cancer cell metabolism A variety of transition state analogues have been synthesized some of which exhibit potent inhibitory activity such as phosphonoacetohydroxamate (1 2) The enzymatic assay described here diverges little

We here report a novel Frster resonance energy transfer (FRET)-based assay for studying the in vitro kinetics of ubiquitination FRET is established upon binding of fluorophore-labeled Ub to eGFP-tagged ZnUBP a domain that exclusively binds unconjugated Ub We name this assay the free Ub sensor system (FUSS) Using Uba1 UbcH5 and CHIP as

Although the field of gene therapy has experienced significant setbacks and limited success it is one of the most promising and active research fields in medicine Interest in this therapeutic modality is based on the potential for treatment and cure of some of the most malignant and devastating diseases affecting humans Over the next decade the relevance of gene therapy to medical

Our peer-reviewed scholarly journals and books have an ever-increasing readership of millions of researchers worldwide Bentham Science currently publishes more than 100 journals in both electronic and printed formats Our journals cover various disciplines in pharmaceutical research and development medical subspecialties engineering technology and social sciences The journals are indexed

STATs (signal transducers and activators of transcription) are a family of latent cytoplasmic proteins that are activated to participate in gene control when cells encounter various extracellular polypeptides Biochemical and molecular genetic explorations have defined a single tyrosine phosphorylation site and in a dimeric partner molecule an Src homology 2 (SH2) phosphotyrosine-binding

Niacin

9 Hwang ES Song SB Nicotinamide is an inhibitor of SIRT1 in vitro but can be a stimulator in cells Cell Mol Life Sci 2017 74(18):3347-3362 10 Morris BJ Seven sirtuins for seven deadly diseases of aging Free Radic Biol Med 2013 56:133-171 11 Fliegert R Bauche A Wolf Perez AM et al 2'-Deoxyadenosine 5'-diphosphoribose is an

Vitamin K is an effective antidote for poisoning with a vitamin K antagonist 42 43 There are 2 distinct enzymatic activities capable of reducing vitamin K1 quinone to the hydroquinone form 44 45 Pathway I is the dithiol-driven activity now known to be catalyzed by VKOR 6 7 whereas pathway II is a reduced NAD phosphate (NADPH)-dependent activity contributed in part by the flavoprotein NADPH

About Chemistry for Biologists Chemistry for Biologists resources aim to help you understand the chemistry and chemical principles that underlie a good deal of biology These resources were hosted on the Chemistry for Biologists website which launched in 2004 and was supported by the Royal Society of Chemistry and the Biochemical Society From 2019 Chemistry for Biologists resources are

Although the field of gene therapy has experienced significant setbacks and limited success it is one of the most promising and active research fields in medicine Interest in this therapeutic modality is based on the potential for treatment and cure of some of the most malignant and devastating diseases affecting humans Over the next decade the relevance of gene therapy to medical

Interleukin 2-regulated In Vitro Antibody Production Following A Single Spinal Manipulative Treatment in Normal Subjects Chiropractic Osteopathy 2010 (Sep 8) 18: 26 The induction and regulation of immune responses involve complex interactions between the immune and nervous systems mediated by the biologic action of numerous humoral factors including neurotransmitters and immunoregulatory

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