hydroxyproline assay cartilage

Cartilage samples derived from adult steer joints also displayed a significant decrease in sGAG biosynthesis after exposure to the pulsed EMF (control group 391 1 107 8 c p m /μg of hydroxyproline 'pulsed EMF' group 262 2 50 9 c p m /μg of hydroxyproline p 0 008 Figure 5b) indicating a 33% decrease in isotope uptake and thus shows the susceptibility to EMF also of adult cartilage Osteoarthritic cartilage had higher F-CHP fluorescence than healthy cartilage Both of these outcomes are consistent with previous studies With the calibration curve the microplate assay was able to absolutely quantify denatured collagen in mechanically damaged rat tail tendon fascicles as reliably as the trypsin-hydroxyproline assay Further

Hydroxyproline Assay Kit

Hydroxyproline Assay Kit works for quantitation of the total collagen of any types and species in tissue specimens and tissue homogenates Pre-coated: No Standard Range: 400 g/ml to 6 3 g/ml Working Time (may vary): 1 Hour Sample Type(s): Tissue Homogenate Cells Collagen Solution Read At: 530 nm References Abu-Tair L et al Natural killer cell-dependent anti-fibrotic pathway in

Cartilage Tissue Enhances Proteoglycan Retention by Nucleus Pulposus Cells In Vitro Claudia J Arana Eleftherios P Diamandis and Rita A Kandel Objective To investigate the effect of cartilage on nucleus pulposus (NP) tissue in an in vitro model Methods Cells were isolated from bovine NP or articular cartilage and allowed to form tissue in vitro The NP tissue was grown either alone or

Tobacco hydroxyproline-rich systemins to the tobacco precursor 12 18 The native peptides are active at low nanomolar concentrations in the alkalinization assay but the synthetic peptides without carbohydrate moieties have greatly reduced activity indicating the importance of glycosylation for their biological activity Because of the low binding affinity of synthetic HypSys peptides

Unlike highly regenerative animals such as axolotls humans are believed to be unable to counteract cumulative damage such as repetitive joint use and injury that lead to the breakdown of cartilage and the development of osteoarthritis Turnover of insoluble collagen has been suggested to be very limited in human adult cartilage The goal of this study was to explore protein turnover in

Assay Genie provides a range of POC rapid tests for COVID-19 detection qPCR assays Toggle menu Welcome to Assay Genie! Select Currency: EUR British Pound Euro Japanese Yen US Dollar +44 (0) 2081237624 Sign in or Register Compare Cart 0 Search Categories Disease Areas

Relationship between Relaxation Component T2 values and

Moduli in Native Cartilage O Irrechukwu1 S von-Thaer1 E Frank2 D Reiter1 A Grodzinsky2 and R Spencer1 using the colorimetric DMMB dye binding assay and the hydroxyproline assay respectively Statistical analysis: Linear regression analysis was performed to define the relationships be tween PG and collagen and the derived MR parameters Results and Discussion Our results showed

abundant structural protein that is a component of skin bone tendons cartilage teeth 3 intertwined helical polypeptide chains that form a superhelical cable (NOT a-helices) with a polyproline helix glycine appears every 3rd residue and the sequence gly-pro-pro is common structure of collagen: gly-pro-pro sequence common in collagen hyp modified version of proline after proline is

The assay employs a proprietary acidic developer solution to accurately measure collagen in hydrolysates without the use of hazardous perchloric acid obviating the need for any special handling and waste-disposal protocols The assay can detect a minimum of 0 5 ug collagen per well in a 96-well format and generates colorimetric results that are virtually identical to those of the classical

CHAPTER 1 Introduction to Cartilage Yang Xia * a Konstantin I Momot b Zhe Chen ac Christopher T Chen d David Kahn a and Farid Badar a a Department of Physics and Center for Biomedical Research Oakland University Rochester MI 48309 USA E-mail: xiaoakland edu b School of Chemistry Physics and Mechanical Engineering Queensland University of Technology (QUT) Brisbane Qld 4001

Hyaluronan (HA hyaluronic acid) is composed of alternating residues of β-D-(1→3) glucuronic acid (GlcA) and β-D-(1→4)-N-acetylglucosamine (GlcNAc) (see Figure 2) Unlike the other glycosaminoglycans hyaluronan does not attach to proteins to form proteoglycans

Hydroxyproline content was evaluated by alkaline hydrolysis of the tissue homogenate and subsequent determination of the free hydroxyproline in hydrolysates For scanning electron microscopy (SEM) seeded construct samples were fixed with 2 5% glutaraldehyde in 0 1 M cacodylate buffer (pH 7 4) for 4 hours at 4C rinsed in cacodylate buffer and postfixed in 1% osmium tetroxide for 1 hour

Levels of amino sugars and hydroxyproline (Hypro) indicators of glycosaminoglycan (GAG) and collagen respectively were measured in 10 tissue-engineered chondral constructs after 4 weeks of culture DNA content was analyzed using the Hoechst 33258 dye assay (Sigma-Aldrich) with a calf thymus DNA standard The concentration of amino

BNC and human articular cartilage explants were cultured with interieukin-1 alpha (IL-1 alpha) and/or oncostatin M (OSM) with or without test reagents Collagen levels were determined by assay of hydroxyproline Collagenase activity was measured using the diffuse fibril assay Results The addition of procollagenase activators matrix metalloproteinase 3 (MMP-3) and APMA to IL-1 alpha /OSM

Amazon: Total Collagen Assay Kit (Perchlorate

Amazon: Total Collagen Assay Kit (Perchlorate-Free): Industrial Scientific Skip to main content Try Prime Hello Sign in Account Lists Account Sign in Account Lists Returns Orders Try Prime Cart Industrial Scientific Go Search Hello Select your

miR-148a on cartilage metabolism of OA chondrocytes Design: OA chondrocytes were transfected with a miRNA precursor for hsa-miR-148a or a miRNA pre-cursor negative control After 3 7 14 and 21 days real-time PCR was performed to examine gene expression levels of aggrecan (ACAN) type I II and X collagen (COL1A1 COL2A1 COl10A1) matrix metallopeptidase 13 (MMP13) a disintegrin and

China Best Quality Firming Agent Dipalmitoyl CAS 51-35-4 L-Hydroxyproline Powder Bulk L-Hydroxyproline in Stock Raw Material L-Hydroxyproline Find details about China L-Hydroxyproline 51-35-4 L-Hydroxyproline from Best Quality Firming Agent Dipalmitoyl CAS 51-35-4 L-Hydroxyproline Powder Bulk L-Hydroxyproline in Stock Raw Material L-Hydroxyproline - Xi′an Sgonek Biological

Similarly hydroxyproline assay–based quantitation of fibrosis showed no reduction after TAC with adult cardiomyocyte-specific deletion of Hsp47 (Supplemental Figure 4A) Histological quantification using the CHP ( 37 ) assay also showed similar accumulation of unfolded collagen content in cardiomyocyte-specific Hsp47 -deleted and control hearts ( Supplemental Figure 4B )

Silk fibroin/cartilage extracellular matrix scaffolds with sequential delivery of TGF-beta3 for chondrogenic differentiation of adipose-derived stem cells Qiang Yang 1 * Bin-Hong Teng 2 * Li-Na Wang 3 Kun Li 2 Chen Xu 2 Xin-Long Ma 1 Yang Zhang 1 De-Ling Kong 3 Lian-Yong Wang 3 Yan-Hong Zhao2 1Department of Spine Surgery Tianjin Hospital Tianjin People's Republic of China 2School and

Hexosamine and hydroxyproline contents were also determined Spectrophotometric analysis was used to quantify any changes in the depth of defects in the cartilage ranging from surface level (red-colored) to the deepest affected layer (blue-colored) Interleukin-1β treatment alone caused significant additional cartilage erosion Electrical stimulation alone resulted in significant decreases in

Prolyl-hydroxyproline (Pro-Hyp) is one of the major constituents of collagen-derived dipeptides We previously reported that Pro-Hyp promotes the differentiation of osteoblasts by increasing Runx2 osterix and Col1α1 mRNA expression levels Here to elucidate the mechanism of Pro-Hyp promotion of osteoblast differentiation we focus on the involvement of Foxo1 in osteoblast differentiation

miR-148a on cartilage metabolism of OA chondrocytes Design: OA chondrocytes were transfected with a miRNA precursor for hsa-miR-148a or a miRNA pre-cursor negative control After 3 7 14 and 21 days real-time PCR was performed to examine gene expression levels of aggrecan (ACAN) type I II and X collagen (COL1A1 COL2A1 COl10A1) matrix metallopeptidase 13 (MMP13) a disintegrin and

Juva K Prockop DJ An effect of puromycin on the synthesis of collagen by embryonic cartilage in vitro J Biol Chem PROCKOP DJ EBERT PS A SIMPLE METHOD FOR DIFFERENTIAL ASSAY OF TRITIUM AND CARBON-14 IN WATER-SOLUBLE BIOLOGICAL MATERIALS Anal Biochem 1963 Sep 6:263–271 Juva K Prockop DJ Modified procedure for the assay of H-3-or C-14-labeled hydroxyproline

Total Collagen Assay Kit II (Perchlorate-Free) is a quick and convenient protocol for detection of small amounts of collagen in a variety of biological samples The assay is based upon hydrolysis of samples to yield free Hyp which is oxidized and reacted to give a brightly-colored chromophore with an absorbance peak at 560 nm The assay employs a proprietary acidic developer solution to

The assay is based on the acid hydrolysis of samples to form hydrolysates and Hydroxyproline This released Hydroxyproline gets oxidized to form a reaction intermediate which further in the reaction forms a chromophore (Abs 560 nm) The assay is simple sensitive and specific for collagen and can detect as low as 0 5 g of collagen in a variety of samples such as tissue homogenates

Copyright © 2014. All rights reserved.
^ Back to Top